Radiologia Brasileira - Publicação Científica Oficial do Colégio Brasileiro de Radiologia

AMB - Associação Médica Brasileira CNA - Comissão Nacional de Acreditação
Idioma/Language: Português Inglês

Vol. 54 nº 5 - Sep. / Oct.  of 2021

REVIEW ARTICLES
Print 

Page(s) 329 to 335

Whole-body magnetic resonance imaging for the diagnosis of metastasis in children and adolescents: a systematic review and meta-analysis

Autho(rs): Simone Gianella Valduga1,a; Gabriele Carra Forte1,b; Ricardo Pessini Paganin1,c; Diego Gomez Abreu2,d; Tássia Machado Medeiros1,e; Klaus Irion3,f; Bruno Hochhegger1,g; Rita Mattiello1,h

PDF English     

Texto em Português English Text

Keywords: Whole body imaging/methods; Magnetic resonance imaging/methods; Neoplasms/diagnostic imaging; Meta-analysis; Pediatrics.

Descritores: Imagem corporal total/métodos; Ressonância magnética/métodos; Neoplasias/diagnóstico por imagem; Metanálise; Pediatria.

Abstract:
Whole-body magnetic resonance imaging (WB-MRI) is a noninvasive imaging method that can be used to diagnose and stage tumors, as well as to assess therapeutic responses in oncology. The objective of this meta-analysis was to evaluate the accuracy of WB-MRI for the diagnosis of metastases in pediatric patients. The following electronic databases were searched: Medline, Embase, Cochrane Central Register of Controlled Trials, Scientific Electronic Library Online, Latin-American and Caribbean Health Sciences Literature, Cumulative Index to Nursing and Allied Health Literature, Web of Science, and ClinicalTrials.gov. All of the selected studies included children and adolescents with histopathological confirmation of a primary tumor. Collectively, the studies included 118 patients ranging in age from 7 months to 19 years. The pooled sensitivity and specificity of WB-MRI were, respectively, 0.964 (95% CI: 0.944–0.978; I2 = 0%) and 0.902 (95% CI: 0.882–0.919; I2 = 98.4%), with an area under the curve (AUC) of 0.991. We found that WB-MRI had good accuracy for the diagnosis of metastases in pediatric patients and could therefore provide an alternative to complete the staging of tumors in such patients, being a safer option because it does not involve the use of ionizing radiation.

Resumo:
A ressonância magnética de corpo inteiro (WB-MRI) é um método de imagem não invasivo que pode ser usado para diagnosticar, estadiar e avaliar a resposta terapêutica em oncologia. O objetivo desta meta-análise foi avaliar a precisão do diagnóstico de WBMRI no diagnóstico de metástases em crianças. Foram pesquisadas as seguintes bastes de dados: Medline, Embase, Cochrane Central Register of Controlled Trials, Scientific Electronic Library Online, Latin-American and Caribbean Health Sciences Literature, Cumulative Index to Nursing and Allied Health Literature, Web of Science, and ClinicalTrials.gov. Todos os estudos incluíram crianças e adolescentes com prova histopatológica de um tumor original. Os estudos selecionados incluíram 118 pacientes com idade variando de 7 meses a 19 anos. A sensibilidade e especificidade combinadas de WB-MRI foram, respectivamente, 0,964 (IC 95%: 0,944–0,978; I2 = 0%) e 0,902 (IC 95%: 0,882–0,919; I2 = 98,4%), com AUC de 0,991. A WB-MRI tem uma boa precisão para o diagnóstico de metástases em pediatria e pode potencialmente fornecer um método alternativo não ionizante mais seguro para completar o estadiamento da doença maligna em crianças.

INTRODUCTION

Cancer has long been considered a major risk factor for death in the pediatric population(1). However, when cancer is diagnosed at an early stage, the chances of cure can be high(1,2). Various imaging methods have been used in order to assess the extent of local and distant disease(3). Ideally, imaging examinations should be rapid, should provide high quality diagnostic information, and should be safe(2,4,5).

Imaging methods that use ionizing radiation, such as X-ray, computed tomography (CT), bone scintigraphy, and positron emission tomography/CT (PET/CT) are still routinely employed(2,4,5). However, exposure to ionizing radiation is a major consideration in pediatric patients with cancer. Recent improvements in magnetic resonance imaging (MRI) hardware and software have dramatically reduced scanning times, thus making MRI more appropriate for use in children(2,4,6).

Whole-body MRI (WB-MRI) is a noninvasive imaging method that can be used to diagnose and stage tumors, as well as to assess therapeutic responses in oncology(7,8). In addition to providing coverage of the entire body, WBMRI produces images with excellent soft tissue contrast and spatial resolution. More importantly, it does not expose patients to radiation, which makes it the ideal method for assessing the primary lesion and systemic spread in pediatric patients. The effectiveness of WB-MRI has been tested in staging patients with known malignancies, as well as in screening patients with genetic predisposition for malignancy(7,8). One recent systematic review assessed the sensitivity of WB-MRI for the detection solely of skeletal metastases in children with primary solid tumors and found promising results(9).

The superior soft tissue contrast and more detailed depiction of bony structures are recognized as advantages of WB-MRI over PET/CT(2,10,11). Although the use of WBMRI in adults as an alternative to PET/CT is well established, its use for the assessment of metastases is not routine, especially in children and adolescents(2).

The objective of this systematic review and meta-analysis was to evaluate the diagnostic accuracy of WB-MRI for metastatic disease in children and adolescents.


MATERIALS AND METHODS

Protocol and registration


This systematic review and meta-analysis was registered with the International Prospective Register of Systematic Reviews (Registration no. CRD42018114271). The protocol was developed in accordance with the Cochrane Collaboration recommendations(12).

Eligibility criteria

We included studies that evaluated metastatic lesions in pediatric patients using WB-MRI and PET/CT. Editorials and review articles were excluded, as were animal studies and studies that did not include an index test. We imposed no restrictions regarding the language or date of publication.

Study selection

The following electronic databases were searched: Medline (via PubMed), Embase, Cochrane Central Register of Controlled Trials, Scientific Electronic Library Online, Latin American and Caribbean Health Sciences Literature (via the Brazilian Regional Library of Medicine), Cumulative Index to Nursing and Allied Health Literature, Web of Science, and Clinical Trials.gov. The Medline search strategy was adopted for all of the databases. Additional references were identified through manual searches

of the bibliographies of the full-text papers retrieved. In addition, we reviewed the gray literature to identify papers authored by leading experts in the field, as well as manually searching the reference lists of other recent systematic reviews.

Data collection

Two of the authors, working independently, reviewed the abstracts and titles of the eligible studies. The full texts of the potentially relevant articles were then evaluated. Disagreements were resolved through discussion with a third investigator.

The following data were extracted by using a standardized instrument: first author; year of publication; study design; study population (number, age, and sex of the patients); rates of mortality and adverse events; dose of fluorodeoxyglucose (FDG) employed; time from FDG administration to scanning; comparator imaging test; patient preparation; test interpretation; interval between index and comparator tests (≤ or > three months); assessors (number, expertise, experience, consensus procedures, and learning effect data); and the number of patients whose results were confirmed by each type of reference.

Risk of bias (quality) assessment

We screened the selected articles for risk of bias. Disagreements were resolved by consensus. Where additional information required review, we reassessed the study after that information had been obtained from the authors of the article in question. The was used in order to assess the methodological quality of the included studies, we employed the Quality Assessment of Diagnostic Accuracy Studies 2 tool(13).

Strategy for data synthesis

Timing and effect measures

A meta-analysis was performed according to the recommendations of the Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy(12). The software package Review Manager 5.3 and R were used to conduct meta-analysis. For each study, 2 × 2 contingency tables consisting of true-positives, false-positives, false-negatives, and true-negatives for metastasis were extracted or reconstructed. Sensitivities, specificities, positive predictive values, negative predictive values, and diagnostic odds ratios with 95% confidence intervals (CIs) were recalculated. Diagnostic criteria and cutoff values for metastasis were also extracted. A sensitivity analysis was performed to assess the robustness of analyses by excluding studies from the overall analysis of heterogeneity. The heterogeneity of the articles was further assessed by visually inspecting forest plots and by performing chi-square tests (values of p < 0.1 indicating heterogeneity). We used the I2 statistic to quantify inconsistencies among the studies, an I2 > 50% being indicative of substantial heterogeneity.


RESULTS

Figure 1 provides an overview of the literature search and study selection process. After duplicates had been removed, there were 283 eligible studies. During the evaluation of the titles and abstracts, we excluded 260 studies, for the following reasons: adult patients were included (n = 4); no index test was included (n = 206); there was no search for metastases (n = 26); the article type was a review article, letter, or editorial (n = 22); or it was an animal study (n = 2). The remaining 23 articles were then read in full. Of those, another 17 articles were excluded: for including adult patients (n = 7); for not including an index test (n = 6); for not searching for metastasis (n = 2); or for being a review article, letter, or editorial (n = 2). The six remaining articles were included in the systematic review, although two were excluded from the meta-analysis: one because the index study was PET only, rather than PET/CT; and one because the specificity and sensitivity values could not be calculated. Hence, only four articles were included in the meta-analysis.


Figure 1. Flow chart of the study selection process.



Characteristics of the studies included in the systematic review and meta-analysis

Table 1 shows the characteristics of the six studies included in the systematic review. The studies included a collective total of 118 patients, ranging in age from seven months to 19 years. All of the studies included children and adolescents with histopathological confirmation of the primary tumor. The mean sample size was 23 (range, 13–31). Of the 118 patients, 45 (66%) were male. Patients in whom there was incongruence between the findings of the two imaging modalities were further evaluated on the basis of the clinical outcomes(14), focal imaging findings(14,16), findings on follow-up evaluation, and expert panel review(15).




Five of the articles evaluated some form of WB-MRI in comparison with FDG-PET/CT(8). Whole body diffusion-weighted imaging with background body signal suppression (DWIBS) was employed in three studies(16–18), fast spin-echo short-tau inversion-recovery (STIR) sequences were used in two studies(14,16)and T1-weighted spin-echo sequences were used in one study(8). The maximum interval between the two examinations was 20 days. Metastases to bone and lymph nodes were found(14–16). Three studies also investigated the diagnostic performance of the examinations for extranodal disease(15,17,18). All WB-MRI studies were interpreted by two radiologists, whereas all PET/CT studies were interpreted by two nuclear medicine specialists.

Findings

The summary estimates obtained from the analysis of WB-MRI were a sensitivity of 0.964 (95% CI: 0.944–0.978; I2 = 0%) and a specificity of 0.902 (95% CI: 0.882–0.919; I2 = 98.4%). Although the heterogeneity of the sensitivity values was low (I2 = 0%), that of the specificity values was high (I2 = 98.4%), with an area under the curve of 0.991 (Figure 2). The study conducted by Ishiguchi et al.(16) was excluded from the heterogeneity analysis because it was found to have a high risk of bias. Thereafter, the heterogeneity of both measures decreased to 0%. In that scenario, WB-MRI identified metastasis at 778 sites in 55 patients.


Figure 2. Forest plots of the sensitivity and specificity of WB-MRI.



The summary estimates obtained from the analysis of FDG-PET/CT were a sensitivity of 0.916 (95% CI: 0.876– 0.947) and specificity of 0.958 (95% CI: 0.942–0.971). The heterogeneity of the sensitivity and specificity values was high (I2 = 85.3% and I2 = 95.4%, respectively). In the sensitivity analysis, performed in order to assess the robustness of our data, excluding studies from the overall analysis of the risk of bias did not decrease the heterogeneity of the sensitivity and specificity values.

Methodological quality assessment

For all of the studies evaluated, the risk of bias was classified as low, because they met the criteria for one or more of the 14 high risk of bias domains. The individual assessments for each study are shown in Table 2.




DISCUSSION

In this meta-analysis, we showed that WB-MRI has good accuracy for the identification of metastases in children and adolescents. This imaging modality could represent a safer option for tumor staging in pediatric patients, because it does not involve the use of ionizing radiation.

Although PET/CT is increasingly being used as the modality of choice for staging neoplasms(19–25), exposure to even small doses of ionizing radiation may increase the risk of later radiation-induced malignancies, particularly in children(26,27). Any PET/CT examination for staging or for the assessment of treatment response should be conducted after a clear evaluation of the risks of radiation exposure relative to the intended benefits. Considerable progress has been made in reducing the radiation dose in pediatric CT. Despite this progress and careful management, radiation exposure is still a cause for concern in pediatric patients. However, there is now evidence that the radiation dose received by children undergoing CT at adult scanning facilities can be up to twice as high as that administered at academic pediatric centers, where it is adjusted for patient size(28).

The study that was excluded in the final analysis because of the high heterogeneity evaluated metastases to bone and lymph nodes(16). The authors of that study reported that WB-MRI had low specificity for the detection of bone metastases. One possible explanation for this finding is the characteristic hyperintense signal of the bone marrow in the spine and pelvic bones of children and adolescents, because of the highly cellular hematopoietic bone marrow in children(29). Ishigushi et al.(16) obtained a number of false-positives results similar to that obtained in healthy children by Müller et al.(29). However, highly cellular hematopoietic bone marrow can be correctly identified when the hyperintense signals are similar to those of the normal hematopoietic bone marrow, which is important to bear in mind when interpreting the WB-MRI findings in pediatric patients(8,30). In addition, although hematopoietic bone marrow shows minimal hyperintensity in comparison with skeletal muscle, tumor infiltration produces a markedly hyperintense signal much greater than that of muscle.

Of the three MRI studies that used diffusion-weighted imaging protocols(16–18), two of them(17,18) also measured the apparent diffusion coefficient (ADC). One possible advantage of applying quantitative ADC cutoffs is that it can eliminate subjectivity and improve specificity in comparison with a purely visual assessment(28). However, it is clear that there is an overlap in ADC values between malignant lymph nodes and normal/reactive lymph nodes, so the optimal ADC cutoff remains unclear and requires further investigation(18).

We observed that the studies that utilized only STIR sequences for detecting bone metastasis(15,28)achieved the best specificity and sensitivity, with no heterogeneity, and that the method had a diagnostic performance similar to that of PET/CT. Coronal STIR sequences are sensitive to soft-tissue and bone abnormalities because of their additive proton density-weighted, T1-weighted, and T2-weighted contrast with inherent fat suppression. Most pathological tissues are proton-rich and have prolonged T1 relaxation and T2 decay times, resulting in high signal intensity on fast STIR images(28). Kumar et al.(14)used coronal STIR parallel acquisition. Studies have also suggested that STIR WB-MRI allows excellent delineation of focal lesions that show inadequate FDG uptake, due to its high tissue contrast(31). Punwani et al.(15) used respiratory- and cardiac-gated axial and coronal STIR half-Fourier rapid acquisition with relaxation enhancement.

Although PET/CT had high sensitivity and specificity, the data were quite heterogeneous. One possible explanation is differences in the timing of the follow-up imaging and in the baseline neoplasms evaluated. The specificity of FDG-PET/CT was lower for the detection of bone metastases than for the detection of lymph node metastases. However, the efficiency of FDG-PET/CT for distinguishing bone metastasis from neuroblastoma was comparable to what has been reported previously(17,32). In 54% of the subjects evaluated by Ishiguchi et al.(16), nuclear medicine scans were taken at least 13 days after the start of treatment, including chemotherapy. The high (26.9%) false-positive rate in that study could therefore be explained by the well-known high uptake of FDG in bone marrow under chemotherapy(33).

The limitations of this meta-analysis and systematic review are related to the small sample sizes in the available studies. However, the number of sites investigated was considered sufficient to support the conclusions. In addition, only one of the studies evaluated the use of WBMRI for the detection of post-treatment disease.

In conclusion, this systematic review and meta-analysis showed that WB-MRI has good accuracy for the diagnosis of metastatic disease in children and adolescents. Because it does not expose patients to ionizing radiation, WB-MRI could represent a safer alternative for cancer staging.


REFERENCES

1. Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68:394–424.

2. Davis JT, Kwatra N, Schooler GR. Pediatric whole-body MRI: a review of current imaging techniques and clinical applications. J Magn Reson Imaging. 2016;44:783–93.

3. Weiser DA, Kaste SC, Siegel MJ, et al. Imaging in childhood cancer: a Society for Pediatric Radiology and Children’s Oncology Group Joint Task Force report. Pediatr Blood Cancer. 2013;60:1253–60.

4. Greer MLC, Voss SD, States LJ. Pediatric cancer predisposition imaging: focus on whole-body MRI. Clin Cancer Res. 2017;23:e6– e13.

5. Guimarães MD, Noschang J, Teixeira SR, et al. Whole-body MRI in pediatric patients with cancer. Cancer Imaging. 2017;17:6.

6. Morone M, Bali MA, Tunariu N, et al. Whole-body MRI: current applications in oncology. AJR Am J Roentgenol. 2017;209:W336– W349.

7. Petralia G, Padhani AR. Whole-body magnetic resonance imaging in oncology: uses and indications. Magn Reson Imaging Clin N Am. 2018;26:495–507.

8. Daldrup-Link HE, Franzius C, Link TM, et al. Whole-body MR imaging for detection of bone metastases in children and young adults: comparison with skeletal scintigraphy and FDG PET. AJR Am J Roentgenol. 2001;177:229–36.

9. Smets AM, Deurloo EE, Slager TJE, et al. Whole-body magnetic resonance imaging for detection of skeletal metastases in children and young people with primary solid tumors – systematic review. Pediatr Radiol. 2018;48:241–52.

10. Ciliberto M, Maggi F, Treglia G, et al. Comparison between wholebody MRI and fluorine-18-fluorodeoxyglucose PET or PET/CT in oncology: a systematic review. Radiol Oncol. 2013;47:206–18.

11. Xu GZ, Li CY, Zhao L, et al. Comparison of FDG whole-body PET/ CT and gadolinium-enhanced whole-body MRI for distant malignancies in patients with malignant tumors: a meta-analysis. Ann Oncol. 2013;24:96–101.

12. Deeks JJ, Wisniewski S, Davenport C. Chapter 4: Guide to the contents of a Cochrane Diagnostic Test Accuracy Protocol. In: Deeks JJ, Bossuyt PM, Gatsonis C, editors. Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy Version 1.0.0. The Cochrane Collaboration, 2013.

13. Whiting PF, Rutjes AWS, Westwood ME, et al. QUADAS-2: a revised tool for the quality assessment of diagnostic accuracy studies. Ann Intern Med. 2011;155:529–36.

14. Kumar J, Seith A, Kumar A, et al. Whole-body MR imaging with the use of parallel imaging for detection of skeletal metastases in pediatric patients with small-cell neoplasms: comparison with skeletal scintigraphy and FDG PET/CT. Pediatr Radiol. 2008;38:953–62.

15. Punwani S, Taylor SA, Bainbridge A, et al. Pediatric and adolescent lymphoma: comparison of whole-body STIR half-Fourier RARE MR imaging with an enhanced PET/CT reference for initial staging. Radiology. 2010;255:182–90.

16. Ishiguchi H, Ito S, Kato K, et al. Diagnostic performance of 18FFDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma. Ann Nucl Med. 2018;32:348–62.

17. Littooij AS, Kwee TC, Barber I, et al. Whole-body MRI for initial staging of paediatric lymphoma: prospective comparison to an FDGPET/ CT-based reference standard. Eur Radiol. 2014;24:1153–65.

18. Latifoltojar A, Punwani S, Lopes A, et al. Whole-body MRI for staging and interim response monitoring in paediatric and adolescent Hodgkin’s lymphoma: a comparison with multi-modality reference standard including 18F-FDG-PET-CT. Eur Radiol. 2019;29:202– 12.

19. Cunningham J, Iyengar S, Sharma B. Evolution of lymphoma staging and response evaluation: current limitations and future directions. Nat Rev Clin Oncol. 2017;14:631–45.

20. Pierce DA, Preston DL. Radiation-related cancer risks at low doses among atomic bomb survivors. Radiat Res. 2000;154:178–86.

21. Etchebehere E, Brito AE, Kairemo K, et al. Is interim 18F-fluoride PET/CT a predictor of outcomes after radium-223 therapy? Radiol Bras. 2019;52:33–40.

22. Tibana TK, Santos RFT, Arão Filho A, et al. Detection of additional primary malignancies: the role of CT and PET/CT combined with multiple percutaneous biopsy. Radiol Bras. 2019;52:166–71.

23. Lee JC, Teles MS. Prevalence of additional primary malignancies detected incidentally on PET/CT. Radiol Bras. 2019;52:348.

24. Pinto EG, Penha D, Irion KL. PET/CT and the solitary pulmonary nodule. Radiol Bras. 2020;53(2):vii–viii.

25. Mourato FA, Brito AET, Romão MSC, et al. Use of PET/CT to aid clinical decision-making in cases of solitary pulmonary nodule: a probabilistic approach. Radiol Bras. 2020;53:1–6.

26. Mathews JD, Forsythe AV, Brady Z, et al. Cancer risk in 680,000 people exposed to computed tomography scans in childhood or adolescence: data linkage study of 11 million Australians. BMJ. 2013;346:f2360.

27. Kleinerman RA. Cancer risks following diagnostic and therapeutic radiation exposure in children. Pediatr Radiol. 2006;36 Suppl 2:121–5.

28. Kellenberger CJ, Epelman M, Miller SF, et al. Fast STIR wholebody MR imaging in children. Radiographics. 2004;24:1317–30.

29. Müller LSO, Avenarius D, Olsen OE. High signal in bone marrow at diffusion-weighted imaging with body background suppression (DWIBS) in healthy children. Pediatr Radiol. 2011;41:221–6.

30. Ricci C, Cova M, Kang YS, et al. Normal age-related patterns of cellular and fatty bone marrow distribution in the axial skeleton: MR imaging study. Radiology. 1990;177:83–8.

31. Schmidt GP, Baur-Melnyk A, Herzog P, et al. High-resolution whole-body magnetic resonance image tumor staging with the use of parallel imaging versus dual-modality positron emission tomography- computed tomography: experience on a 32-channel system. Invest Radiol. 2005;40:743–53.

32. Kushner BH, Yeung HW, Larson SM, et al. Extending positron emission tomography scan utility to high-risk neuroblastoma: fluorine-18 fluorodeoxyglucose positron emission tomography as sole imaging modality in follow-up of patients. J Clin Oncol. 2001;19:3397–405.

33. Sugawara Y, Fisher SJ, Zasadny KR, et al. Preclinical and clinical studies of bone marrow uptake of fluorine-1-fluorodeoxyglucose with or without granulocyte colony-stimulating factor during chemotherapy. J Clin Oncol.. 1998;16:173–80.










1. Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil
2. Universidad Industrial de Santander (UIS), Bucaramanga, Santander, Colombia
3. Thoracic Imaging DIIRM, Manchester University and Manchester University NHS Foundation Trust Department of Radiology, Manchester, UK

a. https://orcid.org/0000-0001-9167-7284
b. https://orcid.org/0000-0002-1480-8196
c. https://orcid.org/0000-0002-7156-0895
d. https://orcid.org/0000-0002-4303-5225
e. https://orcid.org/0000-0002-0536-2110
f. https://orcid.org/0000-0002-7860-7879
g. https://orcid.org/0000-0003-1984-4636
h. https://orcid.org/0000-0002-0548-3342

Correspondence:
Gabriele Carra Forte
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
Avenida Ipiranga, 6690, 1º andar, Petrópolis
Porto Alegre, RS, Brazil, 90610–000
Email: gabicarraforte@yahoo.com.br

Received 8 December 2020
Accepted after revision 15 January 2021

Publication date: 16/04/2021
 
RB RB RB
GN1© Copyright 2021 - All rights reserved to Colégio Brasileiro de Radiologia e Diagnóstico por Imagem
Av. Paulista, 37 - 7° andar - Conj. 71 - CEP 01311-902 - São Paulo - SP - Brazil - Phone: (11) 3372-4544 - Fax: (11) 3372-4554